Cell Culture Reagents
Cellvation Cryopres Medium
Cellvation is a unique cryopreservation medium designed for optimal cell recovery and convenience. It does not contain DMSO or serum, and is scientifically engineered for Serum-Free cultures, as well as, ideally suited for all types of serum. To complete the serum-free product range, MP introduces CellVationtm, a unique cryopreservation medium which does not contain either DMSO or serum. This cell freezing medium offers the following benefits: – No presence of serum during freeze/thaw-cycles of cells adapted to serum-free growth – Absence of cytotoxicity or morphological differentiation associated with DMSO – Optimal recovery and faster cell attachment to substrate – Ready to use CellVation has a proven track record of successful freeze/thaw cycles of over 30 cell types, including primary and finite life cells as well as suspension and anchorage-dependent cell lines.
Endothelial Cell Growth Supplement
From Bovine Hypothalamus
Lyophilized from sodium chloride buffer containing streptomycin sulfate
Activity: promotes the growth of Fetal Bovine Heart Endothelial Cells (FBHEC) in 10% newborn calf serum when reconstituted at a concentration of 50-150 μg/ml serum-free medium.
It contains several growth factors (e.g. FGF, ECGF-α) and supports the growth of vascular endothelial cells from human and other mammalian species. Also, it may be used for the cloning and proliferation of hybridomas and human keratinocytes.
Each lot is sterile filtered and tested for bacteria, fungi, and mycoplasma contamination. Some cells may require supplementation with 1-100 μg/ml heparin.
Epidermal Growth Factor 95%
Epidermal growth factor or EGF is a growth factor isolated from mouse submaxillary glands that stimulates cell growth, proliferation, and differentiation by binding to its receptor EGFR. EGF is mitogenic for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, mammary epithelial cells, vascular and corneal endothelial cells, bovine granulosa, rabbit chondrocytes, HeLa and SV40-3T3 cells.
200 mM L-Glutamine (Frozen)
L-glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. L-glutamine is very stable as a dry powder and as a frozen solution. In liquid media or stock solutions, however, L-glutamine degrades relatively rapidly. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use.